Abstract
Background and aims: This study investigates the kinetics of inhibitory activities of ethyl acetate leaf fraction of Lecanodiscus cupanoides on ๐ผ-amylase and ๐ผ-glucosidase.
Methods: This was done using ๐ผ-amylase and ๐ผ-glucosidase enzymes from Aspergillus oryzae and Saccharomyces cerevisiae respectively, active component and antioxidants capabilities of the fraction were also analyzed.
Results: The fraction scavenged DPPH, OH*, and ABTS+ and chelated Fe2+ with the IC50 values of 0.46, 0.70, 0.75 and 0.97 mg/mL respectively. However, significant and mild inhibitory effect was also observed in ๐ผ- amylase and glucosidase with IC50 values (0.73 and 0.58 mg/mL) in concentration dependent pattern. Kinetic analysis of the fraction revealed an uncompetitive and non-competitive mode of inhibition for ๐ผ-amylase and ๐ผ-glucosidase respectively.
Conclusion: This study suggests that ethylacetate fraction of this plant possesses antidiabetic property as a result of its ability to inhibit the metabolism of carbohydrate hydrolyzing enzymes. The elicited activities of the ethyl acetate fraction Lecaniodiscus cupanoides may be due to the presence of phytochemicals of utmost pharmacological importance present in the fraction.