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Future Nat Prod. 2018;4(2): 33-47.
  Abstract View: 172
  PDF Download: 111

Original article

Kinetics of inhibitory effect of ethyl acetate leaf fraction of Lecaniodiscus cupanoides on enzymes linked to carbohydrate metabolism: An in vitro assessment

Abdulwakeel Ayokun-nun Ajao 1*, Adigun Rasheed Adewale 2, Jimoh Abdullahi Adekilekun 3, Adams Yusuf Agbomekhe 1, Sanni Muideen Adedayo 4

1 Botany Dept., Obafemi Awolowo University, Ile-Ife, Nigeria 220005
2 Pure and Applied Chemistry Dept., Usmanu Danfodiyo University Sokoto, Nigeria
3 Microbiology Dept., University of Kwazulu Natal, Westville Campus, South Africa
4 Chemistry Dept., Obafemi Awolowo University, Ile-Ife, Nigeria 220005
*Corresponding Author: Ajao Abdulwakeel Ayokun-nun. Botany Dept., Obafemi Awolowo University, Ile-Ife, Nigeria 220005, Tel: 00927833171723;, Email: ajwak880@gmial.com

Abstract

Background and aims: This study investigates the kinetics of inhibitory activities of ethyl acetate leaf fraction of Lecanodiscus cupanoides on ๐›ผ-amylase and ๐›ผ-glucosidase.

Methods: This was done using ๐›ผ-amylase and ๐›ผ-glucosidase enzymes from Aspergillus oryzae and Saccharomyces cerevisiae respectively, active component and antioxidants capabilities of the fraction were also analyzed.

Results: The fraction scavenged DPPH, OH*, and ABTS+ and chelated Fe2+ with the IC50 values of 0.46, 0.70, 0.75 and 0.97 mg/mL respectively. However, significant and mild inhibitory effect was also observed in ๐›ผ- amylase and glucosidase with IC50 values (0.73 and 0.58 mg/mL) in concentration dependent pattern. Kinetic analysis of the fraction revealed an uncompetitive and non-competitive mode of inhibition for ๐›ผ-amylase and ๐›ผ-glucosidase respectively.

Conclusion: This study suggests that ethylacetate fraction of this plant possesses antidiabetic property as a result of its ability to inhibit the metabolism of carbohydrate hydrolyzing enzymes. The elicited activities of the ethyl acetate fraction Lecaniodiscus cupanoides may be due to the presence of phytochemicals of utmost pharmacological importance present in the fraction.


How to cite the article: Ajao AA, Adewale AR, Abdullahi Adekilekun J, Agbomekhe AY, Adedayo SM. Kinetics of inhibitory effect of ethyl acetate leaf fraction of Lecaniodiscus cupanoides on enzymes linked to carbohydrate metabolism: An in vitro assessment. Adv Herb Med. 2017; 3(3): 7-21.
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Submitted: 07 Jan 2017
Accepted: 28 Mar 2017
ePublished: 30 Apr 2018
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