Antimicrobial effect of ethanolic, methanolic, and ethyl acetate extracts of green tea against Escherichia coli and Listeria monocytogenes

Document Type : Original Article


1 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

2 Department of Food Sciences and Technology, Zahedan University of Medical Sciences, Zahedan, Iran.

3 PhD of Feed Hygiene, Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

4 Department of clinical biochemistry, Faculity of Medicine, Zabol University of Medical Sciences, Zabol, Iran.

5 Center of Agricultural Biotechnology, University of Zabol, Zabol, Iran


Background and aims: One of the most important bacterial species transmitted through the use of aquatic products is Escherichia coli. The main aim of this study was to investigate the antimicrobial effects of ethanolic, methanolic, and ethyl acetate extracts of green tea on Escherichia coli and Listeria monocytogenes.
Methods: The samples of Escherichia coli and Listeria monocytogenes were purchased from the company and an antibiotic resistance pattern was determined. Finally, the minimum inhibitory concentration and minimum bactericidal concentration of ethanolic, methanolic, and ethyl acetate extracts of green tea were investigated.
Results: The results of this study showed that the lowest inhibitory concentration is related to ethyl acetate extract of green tea against E. coli, while the highest inhibitory concentration is related to methanolic extract of green tea against Listeria monocytogenes.
Conclusion: It is worth mentioning that high concentrations of ethanolic, methanolic, and ethyl acetate can be used as a natural antibacterial in fish products.


Main Subjects

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