Comparing the Therapeutic Effects of 6-Gingerol and Hydro-Alcoholic Extract of Ginger on Polycystic Ovary Syndrome in Wistar rat

Document Type : Original Article

Authors

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Skin Disease and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

3 Department of Animal Sciences, Islamic Azad University, Golpayegan Branch, Isfahan, Iran.

Abstract

Background and aims: PCOS (polycystic ovary syndrome) is the most common endocrine and metabolic disorder characterized by amenorrhea, hyper androgens, hirsutism, chronic anovulation and infertility. The aim of the present study was determining the effects of ginger extract, and 6-gingerol on hormonal levels and ovarian follicles in induced PCOS rats, and comparing the ameliorating effects of these two substances for treatment of PCOS.
Methods: In this experimental research, 42 adult female Wistar rats weighting between 160 g-180 g were divided into six groups of 7 animals. PCOS control that received no injection. PCOS received intraperitoneal injections of 100 mg/kg of ginger extract (for 28 days). Statistical analyses with SPSS, one-way ANOVA, T-test and Duncan test were
used to compare groups.
Results: In comparison with PCOS control, the treatment of PCOS rats with ginger extract (100 and 200 mg/kg) and 6-gingerol (200 and 400 µg/kg) led to significant decrease in LH levels. There was a decrease in FSH levels, but the significant one was only in the 6-gingerol treated group (400 µg/kg). In PCOS treated groups with ginger
extract and 6-gingerol, the serum levels of estradiol decreased significantly compared to control and PCOS control groups (p <0.001). Progesterone levels in PCOS groups injected with ginger extract and 6-gingerol showed a significant increase (p <0.05). In PCOS treated groups with ginger extract and 6-gingerol, testosterone levels decreased
significantly (p <0.001, p <0.01, p <0.05).
Conclusion: 6-gingerol and ginger extract may be a useful treatment for improving the PCOS through reduction of estrogen, testosterone, LH and FSH, and improvement of ovulation. In fact, because of anti-inflammatory and antioxidant properties of ginger components, especially 6-gingerol, they can cause to improve PCOS

Keywords

Main Subjects

References

1. Norman RJ, Dewailly D, Legro RS, Hickey TE. Polycystic ovary syndrome. Lancet. 2007; 370 (9588): 685-97.
2. Ramezani F, Daneshpour M, Hashemi S, Zarkesh M. Relationship between polymorphism of insulin receptor gene, and adiponectin gene with PCOS. Iran J Reprod Med. 2013; 11(3): 185-94.
3. Baliga MS, Haniadka R, Pereira MM, D’Souza JJ, Pallaty PL. Update on the chemopreventive effects of ginger and its phytochemicals. Crit Rev Food Sci Nutr. 2011; 51: 499-523.
4. Haniadka R, Rajeev AG, Palatty PL, Arora R, Baliga MS. Zingiber Officinal(ginger) as an anti-emetic in cancer chemotherapy: A review. J Altern Complement MED. 2012; 18: 440-4.
5. Butt, M.S. and Sultan, M.T. Ginger and its health claims: molecular aspects. Critical Reviews in Food Science and Nutrition. 2011; 51: 383-393.
6. Kim EC, Min JK, Kim TY, Lee SJ, Yang HO, Han S, Kim YM, Kwon YG. [6] -Gingerol, a pungent ingredient of ginger, inhibits angiogenesis in vitro and in vivo. Biochem. Biophys. Res. Commune. 2005; 335 (2): 300-308.
7. Sam S and Dunaif A. Polycystic ovary syndrome: syndrome, XX? Trends Endocrinol Metab. 2003; 14:365–370.
8. Kargar Jahromi H, Kargar Jahromi L, Tavakol koukhdan E, Shafiei Jahromi N, Poorgholami F, Rahmanian E, Pourahmadi M.and Hasanpour Jahromi Z. The Effect of Pomegranate Juice Extract on Hormonal Changes of Female Wistar Rats Caused by Polycystic Ovarian Syndrome, Biomedical & Pharmacology Journal. 2015; 8 (2): 971-7.
9. Ghafurniyan H, Nabiuni M, Karimzadeh L. The effect of green tea on IL-6 and CRP level in model of polycystic ovary syndrome as an inflammatory state. International journal of cellular & molecular biotechnology. 2014 (2014): 12 pages.
10. Zafari F, Minaee B, Amirzargar A, Ahangarpour A, Mousavizadeh K. Effects of chamomile extract on biochemical and clinical parameters in a rat model of polycystic ovary syndrome. J Reprod Infertil. 2010; 11 (3): 169-74.
11. Lewandowski K, C, Cajdler-Luba A, Salata I, Bienkiewicz M, LewinskiA..The utility of the gonadotrophin releasing hormone (GnRH)test in the diagnosis of polycystic ovary syndrome (PCOS). Polish journal of endocrinology. 2011; 62 (2): 120–8.
12. Bremer AA, Miller WL. The serine phosphorylation hypothesis of polycystic ovary syndrome: a unifying mechanism for hyperandrogenemia and insulin resistance. Fertil Steril. 2008; 89: 1039-48.
13. Moret M, Stettler R, Rodeo F, Gaillard RC, Waeber G, Wirthner D, et al. Insulin modulation of luteinizing hormone secretion in normal female volunteers and lean polycystic ovary syndrome patients. Neuroendocrinology. 2009; 89: 131-9.
14. Mukherjee S, Maitra A. Molecular & genetic factors contributing to insulin resistance in polycystic ovary syndrome. Indian J Med Res. 2010; 131: 743-760.
15. Ciechanowska M, Lapot M, Malewski T, Mateusiak K, Misztal T, Przekop F. Effects of GABA (A) receptor modulation on the expression of GnRH gene and GnRH receptor (GnRH-R) gene in the hypothalamus and GnRH-R gene in the anterior pituitary gland of follicular-phase ewes. Anim Reprod Sci. 2009; 111(2-4): 235-48.
16. Wang F, Shing M, Huen Y, Tsang SY, Xue H. Neuroactive flavonoids interacting with GABAA receptor complex. Curr Drug Targets CNS Neurol Disord, 4 (5): 575-85.
17. Singh K B, 2005. Persistent estrous rat models of polycystic ovary disease: an update. Fertility and sterility. 2005; 84 (2): 1228-34.
18. Johnson A L. Ovarian follicle selection and granulosa cell differentiation. Poultry science. 2014; 94 (4): 781-5.
19. Rahmanian F, Hemayat Khah Jahromi V, Kargar H. The effects of ginger hydroalcoholic extract on spermatogenesis process and hormone-pituitary-gonadal axis in immature mice. Journal of Science of Teacher Training University. 2012; 10 (3): 915-22.
20. Murakami A, Takahashi, D, Kinoshita T, et al. Zerombone a Southeast Asian ginger sesguiterpene, markedly suppresses free radical generation, proinflammatory protein production, and cancer cell proliferation accompanied by apoptosis: the alpha, beta unsaturated carbonyl group is a prerequisite. Carcinogenesis. 2002; 23950:795-802.
21. Yogeshwer SH, Sahdeo P, Chitra T, Madhulika S et al.In vitro and in vivomodulation of testosterone mediated alterations in apoptosis related proteins by [6] – gingerol. Mul Nutr Food Res. 2007; 168:1492-1502.
22. Kim H S, Yumkham S, Choi J H, Son G H, Kim K, Ryu S H, Suh P G. Serotonin stimulates GnRH secretion through the c-Src-PLC g1 pathway in GT1–7 hypothalamic cells. Journal of Endocrinology. 2006; 190: 581–91.
23. Hanrahan, J. R., Chebib, M., & Johnston, G. A. R. Flavonoid modulation ofGABAA receptors. British Journal of Pharmacology. 2011; 163: 234–245.
24. Campbell, E. L., Chebib, M., Johnston, G. A. R. The dietary flavonoids apigenin and epigallocatechin gallate enhance the positive modulation by diazepam of the activation by GABA of recombinant GABAA receptors. Biochemical Pharmacology. 2004; 68:1631–8.
25. Khaki A, Khaki A A, Hajhosseini L, Sadeghpour Golzar F, Ainehchi. The anti-oxidant effects of ginger and cinnamon on spermatogenesis dysfunction of diabetes rats. Afr J Tradit Complement Altern MED. 2014; 11 (4): 1-8.
26. Li M, Chen P, Yue Q, Li R, Chu R, Zhang W, Wang H. Pungent ginger components modulate human cytochrome P450 enzymes in vitro. Acta Pharmacol Sin. 2013; 34 (9): 1237-42.
27. Zuo T ,Zhu M, Xu W. Roles of oxidative stress in polycystic ovary syndrome and cancers. Oxidative medicine and cellular longevity. 2016; 2016: 14. Available:https://www.hindawi.com/journals/omcl/2016/8589318/
28. Li X.H, Tran V. H, Attenuation of Proinflammatory Responses by S-[6] -Gingerol via Inhibition of ROS/NF-Kappa B/COX2 Activation in HuH7 Cells, Evidence-Based Complementary and Alternative Medicine.2013; 2013: 8. Available https://www.hindawi.com/journals/ecam/2013/146142.
29. Kim, M., Miyamoto, S., Yasui, Y., Oyama, T., Murakami, A. and Tanaka, T. Zerumbone, a tropical ginger sesquiterpene, inhibits colon and lung carcinogenesis in mice. International journal of cancer. 2009; 124: 264-71.
30. Hegazy H G. Ameliorative effects of ginger and α-lipoic acid on oxidative stress and inflammation in senile female rats. African journal of pharmacy and pharmacology. 2011; 5 (8): 1096-105.
Future Natural Products
Volume 4, Issue 1 - Serial Number 1
January 2018
Pages 31-39

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