Comparison anti-giardia activity of Satureja hortensis alcoholic extract and metronidazole in vitro

Document Type : Original Article


1 Department of Veterinary Parasitology, Abhar Branch, Islamic Azad University, Abhar, Iran

2 Department of Pharmacology, Urmia Branch, Islamic Azad University, Urmia, Iran


Background and aims: Giardiasis is known as intestinal infection and created by the flagellate protozoan Giardia lamblia. Some studies showed that phenolic components, present in plant extracts and essential oils, have anti-Giardia activity. The current study was performed to compare anti-Giardia activity of Satureja hortensis (SH) alcoholic extract and metronidazole in vitro.

Methods: In this Laboratory-experimental study, it was separated cysts from the feces by Bingham procedure with minor modification. The numbers of cysts were calculated with Hemusytumetr and the purified cysts of Giardia lamblia (500 µl) were exposed with 
500 µl of extract at concentrations of 10, 100 and 200 mg/ml and 125 mg/kg of metronidazole for 30, 60 and 120 min. The numbers of dead and live cysts was enumerated with a microscope.

Results: Findings in the currnt studyshowed an increase in anti-Giardia activity of extract at high concentrations with increasing time. SH, at 100 mg/ml killed 53% in 30 min, 68% in 60 min and 78% in 120 min. Also, the extract killed by 62% in 30 min, about 88% in 
60 minutes and 92% in 120 minutes. The drug had similar effects in 60 and 120 minutes, but it killed 83% in 30 minutes.

Conclusion: SH alcoholic extract showed anti-Giardia activity at high rates and more time. Thus, SH extract at 200 mg may be suitable alternative for Metronidazole, without side effects. However, anti-Giardia activity of SH needs to more attentions, especially clinical investigations.


Main Subjects

1. Adam RD. Biology of Giardia lamblia. Clin Microbiol Rev. 2001; 14(3): 447-75.
2. Balazs M, Szatloczky E. Electron microscopic examination of the mucosa of the small intestine in infection due to Giardia lamblia. Pathol Res Pract. 1978; 163(3): 251-60.
3. Al-Mekhlafi MS, Azlin M, Nor Aini U, Shaik A, Sa'iah A, Fatmah MS, et al. Giardiasis as a predictor of childhood malnutrition in Orang Asli children in Malaysia. Trans R Soc Trop Med Hyg. 2005; 99(9): 686-91.
4. Heresi GP, Murphy JR, Cleary TG. Giardiasis. Semin Pediatr Infect Dis. 2000; 11: 89-195.
5. Lebwohl B, Deckelbaum RJ, Green PH. Giardiasis. Gastrointest Endosc. 2003; 57(7): 906-13.
6. Andrade Ed, Leite ICG, Rodrigues VdO, Cesca MG. Parasitoses intestinais: uma revisao sobre seus aspectos sociais, epidemiologicos, clinicos e terapeuticos. Rev APS. 2010; 13(2): 231-40.
7. Campanati L, Monteiro-Leal LH. The effects of the antiprotozoal drugs metronidazole and furazolidone on trophozoites of Giardia lamblia (P1 strain). Parasitol Res. 2002; 88(1): 80-5.
8. Harris JC, Plummer S, Lloyd D. Antigiardial drugs. Appl Microbiol Biotechnol. 2001; 57(5-6): 614-9.
9. Petri WA, Jr. Therapy of intestinal protozoa. Trends Parasitol. 2003; 19(11): 523-6.
10. Hernandez F, Hernandez D, Zamora Z, Diaz M, Ancheta O, Rodriguez S, et al. Giardia duodenalis: Effects of an ozonized sunflower oil product (Oleozon) on in vitro trophozoites. Exp Parasitol. 2009; 121(3): 208-12.
11. Sangster N, Batterham P, Chapman HD, Duraisingh M, Le Jambre L, Shirley M, et al. Resistance to antiparasitic drugs: The role of molecular diagnosis. Int J Parasitol. 2002; 32(5): 637-53.
12. Upcroft P, Upcroft JA. Drug targets and mechanisms of resistance in the anaerobic protozoa. Clin Microbiol Rev. 2001; 14(1): 150-64.
13. Anthony JP, Fyfe L, Smith H. Plant active components- a resource for antiparasitic agents? Trends Parasitol. 2005; 21(10): 462-8.
14. Oliveira DR, Leitao GG, Coelho TS, Silva PE, Lourenço MCS, Leitao SG. Ethnopharmacological versus random plant selection methods for the evaluation of the antimycobacterial activity. Rev Bras Farmacognol. 2011; 21(5): 793-806.
15. Pinto AC, Silva DHS, Bolzani VdS, Lopes NP, Epifanio RdA. Produtos naturais: atualidade, desafios e perspectivas. Quím Nova. 2002; 25(1): 45-61.
16. Baser K, Ozek T, Kirimer N, Tumen G. A Comparative Study of the Essential Oils of Wild and Cultivated Satureja hortensis L. J Essent Oil Res. 2004; 16(5): 422-4.
17. Hajhashemi V, Sadraei H, Ghannadi AR, Mohseni M. Antispasmodic and anti-diarrhoeal effect of Satureja hortensis L. essential oil. J Ethnopharmacol. 2000; 71(1-2): 187-92.
18. Dorman H, Hiltunen R. Fe (III) reductive and free radical-scavenging properties of summer savory (Satureja hortensis L.) extract and subfractions. Food Chem. 2004; 88(2): 193-9.
19. Chorianopoulos N, Kalpoutzakis E, Aligiannis N, Mitaku S, Nychas GJ, Haroutounian SA. Essential oils of Satureja, Origanum, and Thymus species: Chemical composition and antibacterial activities against foodborne pathogens. J Agric Food Chem. 2004; 52(26): 8261-7.
20. Adiguzel A, Ozer H, KiliC H, CetiN B. Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi. Czech J Food Sci. 2007; 25 (2): 81-9.
21. Harris JC, Plummer S, Turner MP, Lloyd D. The microaerophilic flagellate Giardia intestinalis: Allium sativum (garlic) is an effective antigiardial. Microbiology. 2000; 146 Pt 12: 3119-27.
22. Gadelha A, Vidal F, Castro T, Lopes C, Albarello N, Coelho M, et al. Susceptibility of Giardia lamblia to Hovenia dulcis extracts. Parasitol Res. 2005; 97(5): 399-407.
23. Calzada F, Yepez-Mulia L, Aguilar A. In vitro susceptibility of Entamoeba histolytica and Giardia lamblia to plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders. J Ethnopharmacol. 2006; 108(3): 367-70.
24. Moon T, Wilkinson JM, Cavanagh HM. Antiparasitic activity of two Lavandula essential oils against Giardia duodenalis, Trichomonas vaginalis and Hexamita inflata. Parasitol Res. 2006; 99(6): 722-8.
25. Vidal F, Vidal JC, Gadelha AP, Lopes CS, Coelho MG, Monteiro-Leal LH. Giardia lamblia: The effects of extracts and fractions from Mentha x piperita Lin. (Lamiaceae) on trophozoites. Exp Parasitol. 2007; 115(1): 25-31.
26. Bingham AK, Jarroll EL, Jr., Meyer EA, Radulescu S. Giardia sp.: Physical factors of excystation in vitro, and excystation vs eosin exclusion as determinants of viability. Exp Parasitol. 1979; 47(2): 284-91.
27. Farsangi M, Sahebany N, Movahed A, Tahmasebi R, Ryani M. Thymus fatality power effects on Giardia cysts in vitro. Southern Med J. 1996; 4(9): 88-99.
28. Alizadeh A, Ranjbar M, Kashani KM, Taheri MM, Bodaghi M. Albendazole versus metronidazole in the treatment of patients with giardiasis in the Islamic Republic of Iran. East Mediterr Health J. 2006; 12(5): 548-54.
29. Amaral FM, Ribeiro MNS, Barbosa-Filho JM, Reis AS, Nascimento FR, Macedo RO. Plants and chemical constituents with giardicidal activity. Rev Bras Farmacogn. 2006; 16: 696-720.
30. Sadjjadi SM, Rostami J, Azadbakht M. Giardiacidal activity of lemon juice, vinifer and vinegar on Giardia intestinalis cysts. Southeast Asian J Trop Med Public Health. 2006; 37 Suppl 3: 24-7.
31. Rahimi-Esboei B, Gholami S, Azadbakht M, Ziaei H. Effect of Hydroalcholic extract of Artemisia annua on cysts of Giardia lamblia in in vitro. J Mazand Univ Med Sci. 2012; 22(90): 72-80.
32. Mirjana Sii, Nada B. Chemical composition and antimicrobial variability of Satureja montana L. essential oils produced during ontogenesis. J Essen Oil Res. 2004; 16(4): 387-91.
33. Reynolds JEF. Martindale the extra pharmacopoeia. 31th ed. I. ondon: Royal pharmaceutical sociely. 1996; 31: 1059-69.
34. Cristani M, D'Arrigo M, Mandalari G, Castelli F, Sarpietro MG, Micieli D, et al. Interaction of four monoterpenes contained in essential oils with model membranes: Implications for their antibacterial activity. J Agric Food Chem. 2007; 55(15): 6300-8.
35. Lanfredi-Rangel A, Attias M, de Carvalho TM, Kattenbach WM, De Souza W. The peripheral vesicles of trophozoites of the primitive protozoan Giardia lamblia may correspond to early and late endosomes and to lysosomes. J Struct Biol. 1998; 123(3): 225-35.
36. Abodeely M, DuBois KN, Hehl A, Stefanic S, Sajid M, Attias M, et al. A contiguous compartment functions as endoplasmic reticulum and endosome/ lysosome in Giardia lamblia. Eukaryot Cell. 2009; 8(11): 1665-76.