Evaluation of antibacterial effect of sesame oil, olive oil and their synergism on Staphylococcus aureus in vitro

Document Type : Original Article

Authors

1 Students Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran.

2 medical student, Students Research Committee, lorestan University of Medical Sciences,khorramabad, Iran.

3 Assistant professor, Department of Public Health, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Abstract

Background and aims: Nosocomial infections are the main cause of the problems related to health and health care costs. One of the pathogens that causes these infections is Staphylococcus aureus. Its resistance to antibiotics forced researchers to find herbal substitution such as Sesame and Olive. This study was aimed to determine antibacterial effect of sesame oil, olive oil and their synergism on S. aureus. 
Methods: In this experimental study, at first, olive oil and sesame oil was extracted by cold press assay and the mixture of them was prepared with equal proportion of both oils, and then the standard strain of lyophilized S. aureus ATCC 25923 was prepared from the Iranian Research Organization for Science and Technology. To determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the oils, micro-broth dilution method was used. 
Results: This study indicated the amounts of MIC for sesame oil, olive oil and their synergism; 32 mg/ml, 32 mg/ml, 8 mg/ml and the amounts of MBC; 128 mg/ml, 
128 mg/ml, 32 mg/ml, respectively. 
Conclusion: The results showed the same antibacterial activity for olive oil and sesame oil. It also showed that mixture of these oils enhances the growth inhibitory and bactericidal properties of the oils compared to using them individually.

Keywords

Main Subjects

References

1. Singh GS, Panday S. Natural products in discovery of potential and safer antibacterial agents. Opportunity, Challenge Scope Natural Product Med Chem. 2011: 63-102.
2. Alberti C, Brun-Buisson C, Burchardi H, Martin C, Goodman S, Artigas A, et al. Epidemiology of sepsis and infection in ICU patients from an international multicentre cohort study. Intensive Care Med. 2002; 28(2): 108-21.
3. VandenBergh MF, Yzerman EP, van Belkum A, Boelens HA, Sijmons M, Verbrugh HA. Follow-up of Staphylococcus aureus nasal carriage after 8 years: redefining the persistent carrier state. J Clin Microbiol. 1999; 37(10): 3133-40.
4. Sharma D, Rajput J, Kaith B, Kaur M, Sharma S. Synthesis of ZnO nanoparticles and study of their antibacterial and antifungal properties. Thin Solid Films. 2010; 519(3): 1224-9.
5. Morgenstern M, Erichsen C, Hackl S, Mily J, Militz M, Friederichs J, et al. Antibiotic Resistance of Commensal Staphylococcus aureus and Coagulase-Negative Staphylococci in an International Cohort of Surgeons: A Prospective Point-Prevalence Study. PLoS One. 2016; 11(2): e0148437.
6. Organization WH. Antimicrobial resistance global report on surveillance: 2014 summary. WHO; 2014.
7. Cimolai N. MRSA and the environment: implications for comprehensive control measures. Eur J Clin Microbiol Infect Dis. 2008; 27(7): 481-93.
8. Hong X, Qin J, Li T, Dai Y, Wang Y, Liu Q, et al. Staphylococcal protein a promotes colonization and immune evasion of the epidemic healthcare-associated MRSA ST239. Front Microbiol. 2016; 7: 951.
9. Eucharia EN. Isolation and antibiogram of Staphylococcus aureus from patients admitted at Specialist Hospital Okitipupa, Ondo State, Nigeria. Br Microbiol Res J. 2016; 14(6): 42-6.
10. Naimi TS, LeDell KH, Como-Sabetti K, Borchardt SM, Boxrud DJ, Etienne J, et al. Comparison of community- and health care-associated methicillin-resistant Staphylococcus aureus infection. JAMA. 2003; 290(22): 2976-84.
11. Kollef MH, Fraser VJ. Antibiotic resistance in the intensive care unit. Ann Intern Med. 2001; 134(4): 298-314.
12. Sefidgar A, Taghizadeh Armaki M, Pournajaf A, Ardebili A, Omidi S, Abdian Asl A. Evaluation of antimicrobial activity of alcoholic and aqueous extracts from common hop (Humulus lupulus) and oak (Quercus castaneifolia). Eval. 2015; 17(93): 39-46.
13. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12(4): 564-82.
14. Samuel AT, Valentine I. Effect of total aflatoxin on the growth characteristics and chlorophyll level of sesame (Sesamum indicum L.). NY Sci J. 2014; 7: 8-13.
15. Remington JP, Troy DB, Beringer P. Remington: The science and practice of pharmacy. USA: Lippincott Williams and Wilkins; 2006.
16. White G. Vitamin E and Minerals: Nutrition from Nuts. Available from: Allaboutvision. com. Retrieved August. 2006; 20: 36-45.
17. Chang L-W, Yen W-J, Huang SC, Duh P-D. Antioxidant activity of sesame coat. Food chem. 2002; 78(3): 347-54.
18. Ryan E, Galvin K, O'Connor TP, Maguire AR, O'Brien NM. Phytosterol, squalene, tocopherol content and fatty acid profile of selected seeds, grains, and legumes. Plant Foods Hum Nutr. 2007; 62(3): 85-91.
19. Nekozad N, Ashktorab T, Mojab F, Alavi H, Azadeh P. The preventative role of sesame oil on phlebitis induced by anti-neoplastic agents. Ebnesina. 2011; 14(1): 10-6.
20. Rallo P, Dorado G, Martin A. Development of simple sequence repeats (SSRs) in olive tree (Olea europaea L.). Theor Appl Genet. 2000; 101(5-6): 984-9.
21. Shah ZH, Hamooh BT, Daur I, Rehman HM, Alghabari F. Transcriptomics and biochemical profiling: Current dynamics in elucidating the potential attributes of Olive. Curr Issues Mol Biol. 2016; 21: 73-98.
22. Visioli F, Caruso D, Grande S, Bosisio R, Villa M, Galli G, et al. Virgin Olive Oil Study (VOLOS): Vasoprotective potential of extra virgin olive oil in mildly dyslipidemic patients. Eur J Nutr. 2005; 44(2): 121-7.
23. Casal S, Malheiro R, Sendas A, Oliveira BP, Pereira JA. Olive oil stability under deep-frying conditions. Food Chem Toxicol. 2010; 48(10): 2972-9.
24. Amani R, Kariman N, Mojab F, Alavi Majd H, Majidi S. Assessing comparison the effect of cooling gel pads and topical olive oil on the intensity of episiotomy pain in primiparous women. Complement Med J facult Nurs Amp. 2015; 4(4): 977-86.
25. Cougnard-Gregoire A, Merle BM, Korobelnik JF, Rougier MB, Delyfer MN, Le Goff M, et al. Olive oil consumption and age-related macular degeneration: The Alienor Study. PLoS One. 2016; 11(7): e0160240.
26. Pereira AP, Ferreira IC, Marcelino F, Valentao P, Andrade PB, Seabra R, et al. Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrancosa) leaves. Molecules. 2007; 12(5): 1153-62.
27. Paymard A, Salehian T, Moghadam MB, Abbasali MZ, Shahnavazi A, Allahyari E, et al. The effect of local olive oil on prevention of pressure ulcers in patients hospitalized in the intensive care unit of Shahid Beheshti Hospital, Yasuj: A double-blind randomized clinical trial. Jap. 2016; 6 (3): 54-61.
28. Mohammadi T, Hatami M, Mirzaee Sisaabad Y, Hooshiari A, Nejatian M. Formulation of blend oil containing Canola and Sesame Oils without synthetic antioxidants. Iran J Nutr Sci amp; Food Technol. 2014; 9(3): 83-92.
29. Bkayyan M. Antimicrobial effect of ethanol extract of garlic (Allium Sativum) on S. aureus strains resistant to antibiotics. J Plant.2015; 28(1): 34-41.
30. Khaledi M, Heidari-Sureshjani R, Gholipour A, Mardanpour-Shahrekordi E, Roohi Broojeni H. Study of the Antimicrobial effects of the hydroalcoholic extract of Teucrium chamaedrys on the bacteria Streptococcus mutans in vitro. J Shahrekord Univ Med Sci. 2016; 17(2): 61-7.
31. Eivari M, Akbar A, Salehi M, Malek Jafarian M. Antimicrobial activity of rosmarinus officinalis on vancomycin-resistant Staphylococcus aureus isolated from Imam Reza Hospital patients of Mashhad. J Neyshabur Univ Med Sci. 2015; 3(3): 39-45.
32. Behdani M, Ghazvini K, Mohammadzadeh A, Sadeghian A. Antibacterial activity of Henna extracts against Staphylococcus aureus and Pseudomonas aeroginosa. Horizon J Med Sci. 2009; 15(2): 46-51.
33. Mahendra Kumar C, Singh SA. Bioactive lignans from sesame (Sesamum indicum L.): Evaluation of their antioxidant and antibacterial effects for food applications. J Food Sci Technol. 2015; 52(5): 2934-41.
34. Bisignano G, Tomaino A, Lo Cascio R, Crisafi G, Uccella N, Saija A. On the in vitro antimicrobial activity of oleuropein and hydroxytyrosol. J Pharm Pharmacol. 1999; 51(8): 971-4.

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