Bactericidal and Bacteriostatic effect of sesame oil, olive oil and their synergism on Escherichia coli in vitro

Document Type : Original Article

Authors

1 Students Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran.

2 medical student, Students Research Committee, lorestan University of Medical Sciences,khorramabad, Iran.

3 Nursing Student, Student Research Committee, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran.

4 MSc in medical microbiology of Shahrekord University of Medical Sciences, Shahrekord, Iran.

5 Nursing Student, students research committee, Dezful University of Medical Sciences, Dezful, Iran.

Abstract

Background and aims: Escherichia coli is one of the important bacteria in nosocomial infections and its resistance to a wide range of antibiotics caused many problems for doctors and patients. So, it was attempted to find new substitution for these antibiotics. The aim of the present study was to determine the antimicrobial effect of olive oil, sesame oil and their synergism on E. coli.
Methods: In this study, olive oil and sesame oil were extracted by cold press assay and standard strain of lyophilized E. coli ATCC 25922 was prepared from the Iranian Research Organization for Science and Technology. Olive oil, sesame oil and their synergism, with the same proportion, in concentrations of 2 mg/ml to 1024 mg/ml were made individually and their MBC and MIC amounts were determined by broth micro dilution.
Results: This study declared MIC and MBC for olive oil; 16 mg/ml and 64 mg/ml, for sesame oil and their mixture; 64 mg/ml and 265mg/ml, respectively.
Conclusion: The study showed that both oils and their synergism inhibits the growth of bacteria and the antibacterial properties of sesame oil is approximately equal to the mixture of olive oil and sesame oil and although the oils have good antibacterial effect, there was no synergetic effect in these oils.

Keywords

Main Subjects

References

1. Sefidgar A, Taghizadeh Armaki M, Pournajaf A, Ardebili A, Omidi S, Abdian Asl A. Evaluation of antimicrobial activity of alcoholic and aqueous extracts from common hop (Humulus lupulus) and oak (Quercus castaneifolia). Evaluation. 2015; 17(93): 39-46.
2. Rajabpour M, Alikhani MY. MIC determination of Pseudomonas aeruginosa strains were isolated from clinical specimens of patients admitted to educational hospitals in Hamedan (90-91). Iran J Med Microbiol. 2013; 7(3): 18-25.
3. Sadrnia M, Arjomandzadegan M. Comparative study on the effects of Aloe vera extract in clinical strains of Staphylococcus aureus, Klebsiella, Staphylococcus epidermidis and Escherichia coli compared to antibiotics of choice. Arak Med Univ J. 2014; 17(6): 39-46.
4. Abdollahi Kheirabadi S, Najafipour S, Kafilzadeh F, Abdollahi A, Jafari S, Moravej A. Evaluation of Drug Resistance Pattern of Escherichia coli Strains Isolated from Fasa Vali-e-Asr Hospital Patients. J Fasa Univ Med Sci. 2013; 2(4): 273-8.
5. Andersen TE, Khandige S, Madelung M, Brewer J, Kolmos HJ, Møller-Jensen J. Escherichia coli uropathogenesis in vitro: invasion, cellular escape, and secondary infection analyzed in a human bladder cell infection model. Infect Immun. 2012; 80(5): 1858-67.
6. Justice SS, Hunstad DA, Seed PC, Hultgren SJ. Filamentation by Escherichia coli subverts innate defenses during urinary tract infection. Proc Natl Acad Sci. 2006; 103(52): 19884-9.
7. Malakootian M, Hatami B. Survey of chemical composition and antibacterial activity of Rosmarinus officinalis essential oils on Escherichia coli and Its Kinetic. 2013; 12(1): 1-13.
8. Boniadian M, Habibian R, Barati S, Jostejo T. Investigation the antibiotic resistance of the Escherichia coli isolated from gastroenteritis cases in Shahrekord county. J Shahrekord Univ Med Sci. 2013; 15(6): 117-23.
9. Meshkibaf MH, Abdollahi A, Fasihi Ramandi M, Adnani Sadati SJ, Moravvej A, Hatami S. Antibacterial effects of hydro-alcoholic extracts of Ziziphora tenuior, Teucrium polium, Barberis corcorde and Stachys inflate. Koomesh. 2010; 11(4): 240-4.
10. Behdani M, Ghazvini K, Mohammadzadeh A, Sadeghian A. Antibacterial activity of Henna extracts against Staphylococcus aureus and Pseudomonas aeroginosa. J Horizon Med Sci. 2009; 15(2): 46-51.
11. Eisenberg DM, Davis RB, Ettner SL, Appel S, Wilkey S, Van Rompay M, et al. Trends in alternative medicine use in the United States, 1990-1997: Results of a follow-up national survey. Jama. 1998; 280(18): 1569-75.
12. Amani R, Kariman N, Mojab F, Alavi Majd H, Majidi S. Assessing comparison the effect of cooling gel pads and topical olive oil on the intensity of episiotomy pain in primiparous women. Complement Med J faculty of Nurs. 2015; 4(4): 977-86.
13. Eidi A, Moghadam-kia S, Zarringhalam Moghadam J, Rezazadeh S, Eidi M. Antinociceptive effect of olive oil (Olea europaea L.) on mice. Arak Med Univ J. 2011; 14(4): 52-9.
14. Cicerale S, Lucas LJ, Keast RS. Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive oil. Curr Opin Biotechnol. 2012; 23(2): 129-35.
15. Cicerale S, Lucas L, Keast R. Biological activities of phenolic compounds present in virgin olive oil. Int J Mol Sci. 2010; 11(2): 458-79.
16. Bagheri M, Molzemi S, Hadizadeh M, Aminian M. Study of Alcohol Extract of Sesame on wound healing of diabetic male wistar rats. J Neyshabur Univ Med Sci. 2016; 3(4): 1-9.
17. Kiran K, Asad M. Wound healing activity of Sesamum indicum L seed and oil in rats. Indian J Exp Biol. 2008; 46(11): 777-82.
18. Shittu L, Bankole M, Ahmed T, Aile K, Akinsanya M, Bankole M, et al. Differential antimicrobial activity of the various crude leaves extracts of Sesame radiatum against some common pathogenic micro-organisms. Sci Res Essay. 2006; 1(3): 108-11.
19. Heidari-Soureshjani R, Obeidavi Z, Reisi-Vanani V, Ebrahimi Dehkordi S, Fattahian N, Gholipour A. Evaluation of antibacterial effect of sesame oil, olive oil and their synergism on Staphylococcus aureus in vitro. Adv Herbal Med. 2016; 2(3): 13-9.
20. Were BA, Onkware AO, Gudu S, Welander M, Carlsson AS. Seed oil content and fatty acid composition in East African sesame (Sesamum indicum L.) accessions evaluated over 3 years. Field Crops Res. 2006; 97(2): 254-60.
21. Mohammadi T, Hatami M, Mirzaee Sisaabad Y, Hooshiari A, Nejatian M. Formulation of Blend Oil Containing Canola and Sesame Oils without Synthetic antioxidants. Iran J Nutr Sci. 2014; 9(3): 83-92.
22. Soureshjani RH, Derakhshan A, Khaledi M, Gholipour A. Ibactericidal and bacteriostatic in vitro effects of teucrium chamaedrys hydroalcoholic extract on two bacterial causative agents of tooth decay; 2008.
23. Khaledi M, Heidari SR, Gholipour A, Mardanpour SE, Roohi Broojeni H. Study of the antimicrobial effects of the hydroalcoholic extract of Teucriumchamaedrys on the bacteria Streptococcus mutans in vitro. J Shahrekord Univ Med Sci. 2016; 17(2): 61-7.
24. Bonjar GH. Antibacterial screening of plants used in Iranian folkloric medicine. Fitoterapia. 2004; 75(2): 231-5.
25. Izadi Z, Esna-Ashari M, Ahmadvand G, Davoodi P, Piri K. Chemical composition and antibacterial activity of the essence oil of peppermint (Mentha piperita L). Armaghane danesh. 2009; 14(3): 45-54.
26. Sudjana AN, D'Orazio C, Ryan V, Rasool N, Ng J, Islam N, et al. Antimicrobial activity of commercial Olea europaea (olive) leaf extract. Int J Antimicrob Agents. 2009; 33(5): 461-3.
27. Pereira AP, Ferreira IC, Marcelino F, Valentao P, Andrade PB, Seabra R, et al. Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrancosa) leaves. Molecules. 2007; 12(5): 1153-62.
28. Aziz NH, Farag SE, Mousa LA, Abo-Zaid MA. Comparative antibacterial and antifungal effects of some phenolic compounds. Microbios. 1998; 93(374): 43-54.
29. Osibote E, Ogunlesi M, Okiei W, Asekun T, Familoni O. Assessment of antimicrobial activity of the essential oil from the stem powder of Cissus populnea and the leaves of Sesamum radiatum, herbal medications for male infertility factor. J Med Plants Res. 2010; 4: 14-20.
30. Mahendra Kumar C, Singh SA. Bioactive lignans from sesame (Sesamum indicum L.): Evaluation of their antioxidant and antibacterial effects for food applications. J Food Sci Technol. 2015; 52(5): 2934-41.

Files

Share

How to cite

Statistics