Efficacy of hydroalcoholic Petroselinum crispum L. leaf extract on pentylenetetrazole-induced seizure in rats

Document Type : Original Article

Authors

1 Deputy of Research and Technology, Shahrekord University of Medical Science, Shahrekord, Iran

2 Student Research Committee, Shahrekord University of Medical Science, Shahrekord, Iran

3 Medical Plants Research Center, Shahrekord University of Medical Science, Shahrekord, Iran

Abstract

Background and aims: Epilepsy is a disorder of the central nervous system that manifests with sudden, transient, recurrent and unpredictable seizures of sensory-motor, autonomic origin. Drugs used to treat the disorder may cause numerous side effects and treatment response may be unsatisfactory. The purpose of the present study was to investigate the in vitro effects of hydroalcoholic Petroselinum crispum L. leaf extract on pentylenetetrazole (PTZ)-induced seizure in rats.
Methods: In this experimental study, 60 male rats were randomly divided into 6 groups of 10 each. Control group received normal saline. Model group received PTZ at 90 mg/kg intraperitoneally. Intervention groups received P. crispum extract at concentrations of 100, 150 and 200 mg/kg 30 minutes before PTZ administration. Positive control group received 40 mg/kg phenobarbital 30 minutes before PTZ injection. Then, seizure threshold was recorded. In addition, serum and brain antioxidant capacity and malondialdehyde (MDA) levels were measured.
Results: Treatment of mice given PTZ with different concentrations of P. crispum extract caused a significant increase in seizure threshold (P < 0.05). In mice receiving PTZ, a significant increase in serum and brain MDA levels was observed (P < 0.05) but no significant change in antioxidant capacity was noticed. Treatment of mice given PTZ with different concentrations of the extract led to a significant increase in brain and serum antioxidant capacity and a significant decrease in brain and serum MDA levels (P < 0.05).
Conclusion: P. crispum shows protective efficacy against PTZ-induced seizures, which may be due to its antioxidant effects.

Keywords


1.Bourgeois BF, Dodson E. Pediatric epilepsy: diagnosis and therapy. 3rd ed. New York; Demos Medical Publishing: 2007.
2.Shorvon S. Handbook of epilepsy treatment. 3rd ed. London; John Wiley & Sons: 2010
3.Mirazi N, Shamohammadi S, Hosseini A. Effects of Malva silvestris hydroethanolic extract on seizure induced by intravenous injection of pentylenetetrazole in male mice. Zanjan Uni Med J. 2015; 23(101): 98-107.
4.Zhang H, Chen F, Wang X, Yao H-Y. Evaluation of antioxidant activity of parsley (Petroselinum           crispum) essential oil and identification of its antioxidant constituents. Food Res Int. 2006; 39(8): 833-9.
5.Vora SR, Patil RB, Pillai MM. Protective effects of Petroselinum crispum (Mill) Nyman ex AW Hill leaf extract on D-galactose-induced oxidative stress in mouse brain. Indian J Exp Biol. 2009; 48(1): 338-43.
6.Haidari F, Keshavarz SA, Shahi MM, Mahboob S-A, Rashidi M-R. Effects of parsley (Petroselinum crispum) and its flavonol constituents, kaempferol and quercetin, on serum uric acid levels, biomarkers of oxidative stress and liver xanthine oxidoreductase aactivity inoxonate-induced hyperuricemic rats. Iran J Pharm Res. 2011; 10(4): 811-12.
7.Haidari F, Keshavarz SA, Shahi MM, Mahboob S-A, Rashidi M-R. Effects of parsley (Petroselinum crispum) and its flavonol constituents, kaempferol and quercetin, on serum uric acid levels, biomarkers of oxidative stress and liver xanthine oxidoreductase aactivity inoxonate-induced hyperuricemic rats. Iran J Pharm Res. 2011; 10(4): 811-12.
8.Eidi A, Eidi M, Badiei L. Antinociceptive effects of ethanolic extract of parsley (Petroselinum crispum L.) leaves in mice. Med Sci J Islamic Azad Univ. 2009; 19(3): 55-9.
9.Ziai SA, Moradganjeh A. Effects of aqueous extract of parsley on prevention of nerve cells peroxidation in an experimental model of Parkinson’s disease induced by 6-hydroxy dopamine in male rats. Res Med. 2017; 41(1): 17-23.
10.Rostampour M, Aghaei I, Soltani B, Khakpour B. Effect of Matricaria chamomilla hydro-alcoholic extract on ptz-induced seizure in male mice. J Guilan Univ Med Sci. 2014; 23(89): 8-14
11.El-Sherbiny DA, Khalifa AE, Attia AS, Eldenshary EE-DS. Hypericum perforatum extract demonstrates antioxidant properties against elevated rat brain oxidative status induced by amnestic dose of scopolamine. Pharmacol Biochem Behav. 2003; 76(3): 525-33
12.Chaves D, Frattani FS, Assafim M, Costa S. Phenolic chemical composition of Petroselinum crispum extract and its effect on haemostasis. Nat Prod Commun. 2011; 6(7): 961-4.
13.Losi G, Puia G, Garzon G, de Vuono MC, Baraldi M. Apigenin modulates GABAergic and glutamatergic transmission in cultured cortical neurons. Europ J Pharmacol. 2004; 502(1): 41-6.
14.Lin TY, Lu CW, Chang CC, Huang SK, Wang SJ. Luteolin inhibits the release of glutamate in rat cerebrocortical nerve terminals. J Agric Food Chem. 2011; 59(15) :8458-66.
15.Zarei M, Mohammadi S, Shahidi S, Fallahzadeh AR. Effects of Sonchus asper and apigenin-7-glucoside on nociceptive behaviors in mice. J Pharm Pharmacognos Res. 2017; 5(4): 217-27.
16.Fejes S, Blazovics A, Lemberkovics E, Petri G, Szöke E, Kery A. Free radical scavenging and membrane protective effects of methanol extracts fromAnthriscus cerefolium L.(Hoffm.) andPetroselinum crispum (Mill.) Nym. ex AW Hill. Phytother Res. 2000; 14(5): 362-5.
17.Nielsen S, Young JF, Daneshvar B, Lauridsen S, Knuthsen P, Sandström B, et al. Effect of parsley (Petroselinum crispum) intake on urinary apigenin excretion, blood antioxidant enzymes and biomarkers for oxidative stress in human subjects. Br J Nutr. 1999; 81(6): 447-55
18.Bradford H. Glutamate, GABA and epilepsy. Prog Neurobiol. 1995; 47(6): 477-511.
19.Han Y, Zhang T, Su J, Zhao Y, Li X. Apigenin attenuates oxidative stress and neuronal apoptosis in early brain injury following subarachnoid hemorrhage. J Clin Neurosci. 2017; 40(21): 157-62.
20.Qiusheng Z, Yuntao Z, Rongliang Z, Dean G, Changling L. Effects of verbascoside and luteolin on oxidative damage in brain of heroin treated mice. Int J Pharmaceut Sci. 2005; 60(7): 539-43
21.Nazari QA, Kume T, Takada-Takatori Y, Izumi Y, Akaike A. Protective effect of luteolin on an oxidative-stress model induced by microinjection of sodium nitroprusside in mice. J Pharmacol Sci. 2013; 122(2): 109-17.
22.Vezzani A. Inflammation and epilepsy. Epilepsy Curr. 2005; 5(1): 1-6.
23.Vezzani A, Granata T. Brain inflammation in epilepsy: experimental and clinical evidence. Epilepsia. 2005; 46(11): 1724-43
24.Vezzani A, French J, Bartfai T, Baram TZ. The role of inflammation in epilepsy. Nat Rev Neurol. 2011; 7(1): 31-8.
25.Kalueff AV, Lehtimaki KA, Ylinen A, Honkaniemi J, Peltola J. Intranasal administration of human IL-6 increases the severity of chemically induced seizures in rats. Neurosci lett. 2004; 365(2): 106-10.
26.Golechha M, Chaudhry U, Bhatia J, Saluja D, Arya DS. Naringin protects against kainic acid-induced status epilepticus in rats: evidence for an antioxidant, anti-inflammatory and neuroprotective intervention. Biol Pharmaceut Bull. 2011; 34(3): 360-5.
27.Al-Howiriny T, Al-Sohaibani M, El-Tahir K, Rafatullah S. Preliminary evaluation of the anti-inflammatory and anti-hepatotoxic activities of'parsley'petroselinum crispum in rats. J Nat Rem. 2003; 3(1): 54-62.
28.Funakoshi-Tago M, Nakamura K, Tago K, Mashino T, Kasahara T. Anti-inflammatory activity of structurally related flavonoids, Apigenin, Luteolin and Fisetin. Int Immunopharmacol. 2011; 11(9): 1150-9.
29.Lee J-H, Zhou HY, Cho SY, Kim YS, Lee YS, Jeong CS. Anti-inflammatory mechanisms of apigenin: inhibition of cyclooxygenase-2 expression, adhesion of monocytes to human umbilical vein endothelial cells, and expression of cellular adhesion molecules. Arch Pharmacal Res. 2007; 30(10): 1318-27.
30.Wang J, Liu Y-T, Xiao L, Zhu L, Wang Q, Yan T. Anti-inflammatory effects of apigenin in lipopolysaccharide-induced inflammatory in acute lung injury by suppressing COX-2 and NF-kB pathway. Inflammation. 2014; 37(6): 2085-90.
31.Fuchs J, Milbradt R. Skin anti-inflammatory activity of apigenin-7-glucoside in rats. Arzn Forsch. 1993; 43(3): 370-2.
32.Fylaktakidou KC, Hadjipavlou-Litina DJ, Litinas KE, Nicolaides DN. Natural and synthetic coumarin derivatives with anti-inflammatory/antioxidant activities. Curr Pharmaceut Des. 2004; 10(30): 3813-33.