Antifungal effects of the extract of the Withania somnifera on Candida albicans

Document Type : Original Article

Authors

1 Zabol Medicinal Plant Research Center, Zabol University of Medical Sciences,Zabol, Iran.

2 Zabol University of Medical Sciences, Zabol, I.R. Iran

3 Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

4 Department of Biology- Faculty of Scienc- Shahid Bahonar University of Kerman- Kerman- Iran.

Abstract

Background and aims: Withania somnifera (W. somnifera), commonly known as Ashwagandha, is an important medicinal plant that has been used in Ayurvedic and indigenous medicine for over 3,000 years. Candidiasis is one of the most common opportunistic fungal diseases in humans. In fact, the most important fungal disease in women is vaginal candidiasis. This study aimed to investigate the antifungal effects of the extracts of the W. somnifera on Candida albicans (C. albicans).
Methods: In this experimental research, 9 vaginal samples were collected using the sterile swap and Falcon tube by the gynecological specialists. The extracts of the shallot and artichoke were prepared using a rotary device. The inhibitory concentration against C. albicans was determined using incubation in media.
Results: The results of this study suggested that the minimum inhibitory concentration (MIC) against C. albicans, which is equivalent 50 ppm to 250 ppm has the highest concentration of inhibitor.
Conclusion: The results of this study showed that the antifungal activity of wind cheese against C. albicans is good, so it can be used as a drug to treat infections caused by C. albicans.

Keywords

Main Subjects

References

1. Deans SG, Ritchie GA. Antimicrobial properties of plant essential oils. Int J Food Microbiol. 1987; 5(1): 165-80.
2. Janssen AM, Scheffer JJ, Baerheim Svendsen A. Antimicrobial activity of essential oils: a 1976-1986 literature review. Aspects of the test methods. Planta Med. 1987; 53(5): 395-8.
3. Dixon DM, McNeil MM, Cohen ML, Gellin BG, La Montagne JR. Fungal infections: A growing threat. Public Health Rep. 1996; 111(3): 226-35.
4. Gow NA, Brown AJ, Odds FC. Fungal morphogenesis and host invasion. Curr Opin Microbiol. 2002; 5(4): 366-71.
5. Lo HJ, Kohler JR, DiDomenico B, Loebenberg D, Cacciapuoti A, Fink GR. Nonfilamentous C. albicans mutants are avirulent. Cell. 1997; 90(5): 939-49.
6. Mitchell AP. Dimorphism and virulence in Candida albicans. Curr Opin Microbiol. 1998; 1(6): 687-92.
7. Saville SP, Lazzell AL, Monteagudo C, Lopez-Ribot JL. Engineered control of cell morphology in vivo reveals distinct roles for yeast and filamentous forms of Candida albicans during infection. Eukaryot Cell. 2003; 2(5): 1053-60.
8. Staab JF, Bradway SD, Fidel PL, Sundstrom P. Adhesive and mammalian transglutaminase substrate properties of Candida albicans Hwp1. Science. 1999; 283(5407): 1535-8.
9. Fu Y, Ibrahim AS, Sheppard DC, Chen YC, French SW, Cutler JE, et al. Candida albicans Als1p: An adhesion that is a downstream effector of the EFG1 filamentation pathway. Mol Microbiol. 2002; 44(1): 61-72.
10. Calderone RA, Fonzi WA. Virulence factors of Candida albicans. Trends Microbiol. 2001; 9(7): 327-35.
11. Bhattacharya SK, Muruganandam AV. Adaptogenic activity of Withania somnifera: An experimental study using a rat model of chronic stress. Pharmacol Biochem Behav. 2003; 75(3): 547-55.
12. Mirjalili MH, Moyano E, Bonfill M, Cusido RM, Palazon J. Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules. 2009; 14(7): 2373-93.
13. Mehrotra V, Mehrotra S, Kirar V, Shyam R, Misra K, Srivastava AK, et al. Antioxidant and antimicrobial activities of aqueous extract of Withania somnifera against methicillin-resistant Staphylococcus aureus. J Microbiol Biotechnol Res. 2011; 1: 40-5.
14. Arora S, Dhillon S, Rani G, Nagpal A. The in vitro antibacterial/synergistic activities of Withania somnifera extracts. Fitoterapia. 2004; 75(3-4): 385-8.
15. Owais M, Sharad KS, Shehbaz A, Saleemuddin M. Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomedicine. 2005; 12(3): 229-35.
16. Bokaeian M, Saeidi S, Shahi Z, Sahraei S, Zarei H, Sohil Baigi G. Evaluation of Antibacterial Activity of Withania somnifera Leaf Extracts against Antibiotic-Resistant Isolates of Klebsiella pneumoniae. Int J Infect. 2014; 1(2): e21085.
17. Pujari S, Gandhi M. Studies on effect of root extracts of Withania somnifera on some clinically isolated bacterial pathogens. J Environ Res Develop. 2012; 7(2A): 1032-5.
18. Singh E, Kamble S, Bipinraj N, Jagtap S. Medicinal plants used by the thakar tribes in Raigad district, Maharashtra for the treatment of snake-bite and scorpion-bite. Int J Phytother Res. 2012; 2(2): 26-35.
19. Singh G, Kumar P. Evaluation of antimicrobial efficacy of flavonoids of Withania somnifera L. Indian J Pharm Sci. 2011; 73(4): 473-8.
20. Singariya P, Kumar P, Mourya KK. Evolution of Indian Ginseng against Different Bacteria and Fungi. Asian J Pharm clin Res. 2012; 5(2): 145-8.
21. Singariya P, Mourya KK, Kumar P. Comparative Microcidal Activity of Withania somnifera and Cenchrus setigerus against the Pathogenic Microorganisms. Int J Pharm Pharm Sci. 2011; 3(55): 511-5.
22. Bisht P, Rawat V. Antibacterial activity of Withania somnifera against Gram-positive isolates from pus samples. Ayu. 2014; 35(3): 330-2.
23. Pandit S, Chang KW, Jeon JG. Effects of Withania somnifera on the growth and virulence properties of Streptococcus mutans and Streptococcus sobrinus at sub-MIC levels. Anaerobe. 2013; 19: 1-8.
24. Kambiz L, Afolayan A. Extracts from Aloe ferox and Withania somnifera inhibit Candida albicans and Neisseria gonorrhea. Afr J Med Med Sci. 2008; 7(1):58-60.
25. Mwitari PG, Ayeka PA, Ondicho J, Matu EN, Bii CC. Antimicrobial activity and probable mechanisms of action of medicinal plants of Kenya: Withania somnifera, Warbugia ugandensis, Prunus Africana and Plectrunthus barbatus. PLoS One. 2013; 8(6): e65619.
26. Baskaran C, Velu S. Phytochemical analysis and in-vitro antimicrobial activity of Withania somnifera (Ashwagandha). J Nat Prod Plant Resour. 2012; 2(6): 711-6.
27. Sundaram S, Dwivedi P, Purwar S. In vitro evaluation of antibacterial activities of crude extracts of Withania somnifera (Ashwagandha) to bacterial pathogens. Asian J Biotechnol. 2011; 3(2): 194-9.
Future Natural Products
Volume 3, Issue 1 - Serial Number 1
January 2017
Pages 31-37

Files

Share

How to cite

Statistics