Study the antimicrobial effects of artemisia santonica extract on some pathogenic bacteria

Document Type : Original Article


1 Department of Biology- Faculty of Scienc- Shahid Bahonar University of Kerman- Kerman- Iran.

2 Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman


Background and aim: For rapid growing drug resistance among pathogenic microorganisms, it is essential to find new compounds with antimicrobial activity. Medicinal herbs application in traditional treatment for thousand years, make this natural compound to the best choice for antimicrobial studies. The aim of this study is to determine the antimicrobial activity of Artemisia against some important pathogenic bacteria. Material and Method: In the present study, antimicrobial activity of ethanolic and methanolic extracts of A. santonica against four pathogenic bacteria was evaluated. A. santonica dry powder was extracted with ethanol and methanol solvents in maceration method. Assessment of antimicrobial efficacies of extracts and MIC were conducted by disk diffusion manual and macro broth dilution method, respectively. Results: Both ethanolic and methanolic extracts could inhibit microorganism in this test. Staphylococcus aureus with MIC=1.56 mg/ml indicates more sensitive to the Artemisia. In addition, Bacillus cereus showed medium sensitivity. E.coli and Pseudomonas aeruginosa became involved in less sensitivity to the A. santonica extracts. Conclusion: The result of this study confirmed that A. santonica extracts were the best inhibitory effect on S. aureus. Then it can be concluded that by using herbal plant against antibiotics some antibiotic resistant problem can be resolved.


Main Subjects

1. Parekh J, Chanda S. In vitro antimicrobial activity of Trapanatans fruit rind extracted in different solvents. Afr J Biotechnol. 2007; 6(6): 766-70.
2. Yun KW, Mauri MA. Allelopatic potential of Artemisia campestris. Can J Bot. 1997; 75(11): 1903-12.
3. Hassawi D, Kharma A. Antimicrobial activity of some medicinal plants against Candida albicans. J Biol Scien. 2006; 6(1): 109-14.
4. Hakimi MH, Afkhami M, Myrjalili F. An investigation into biological activities of Artemisia persica’s essential oil. Pajouhesh and Sazandegi. 2003; 61: 2-5.
5. Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol. 2001; 74(2): 113-23.
6. Andrews JM. BSAC standardized disc susceptibility testing method. J Antimicrob Chemother. 2001; 48( Suppl 1): 43-57.
7. Baron EJ, Finegold SM. Methods for testing antimicrobial effectiveness, Baily and Scott`s diagnostic microbiology. NewYork: Mosby; 1994.
8. Erel SB, Reznicek G, Şenol SG, Yavaşoğulu NÜK, Konyalioğlu S, Zeybek AU. Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia. Turk J Biol. 2012; 36(1): 75-84.
9. Hendi E, Sefidkon F, Yousefi M, Teimouri M. Essential oil composition and antimicrobial activities of oil, alcoholic extract of Artemisia sieberi from Firoozkooh region. Iran J Biol. 2012; 25(3): 445-55.