Extraction and measurement of the Quercetin flavonoid of Prosopis farcta in Khouzestan climatic condition

Document Type : Original Article

Authors

1 Student, Ramin University of Agriculture and Natural Resources, Ahwaz, I.R. Iran

2 Institute of Medicinal Plants, Karaj, I.R. Iran

3 Agronomy Dept., Ramin University of Agriculture and Natural Resources, Ahwaz, I.R. Iran

4 Psychosocial Injuries Research Center, Ilam University of Medical Sciences, Ilam, I.R. Iran

Abstract

Background and aims: Medicinal plants are a valuable resource for flavonoids extraction. Prosopis fracta is one of the plants with medicinal properties. Prosopis fracta was found in abundance in southern regions in Iran. Coersetin is medicinal substance found in the fruit of this plant. Quercetin is used in treatment of cancer and viral infections. This study was conducted to determine the Quercetin flavonoid in Prosopis fracta samples in different regions of Khuzestan. Methods: The Prosopis fracta fruit collected from different regions of Khuzestan (Susangerd, Ahvaz, Abadan, Mollasani, Behbahan and Ramhormoz). The beads were isolated from the fruit. Shell and the flesh were dried in an Oven. The dried materials were mixed and flavonoids extracted with a suitable solvent. The extract was injected into the High Performance Liquid Chromatography (HPLC) system. Then, the compound, Quercetin quantity and standard peak in each sample have been determined. Results: Based on the results, the Susangerd samples (0.0033 mg/ml) and Abadan (0.0008 mg/ml) have maximum and minimum levels of Quercetin flavonoid, respectively. Conclusions: Quercetin flavonoid extracted from Prosopis farcta fruits of Susangerd is richer than other regions of Khuzestan province. Therefore, it is recommended to use the Prosopis fracta fruit grown in Susangerd for extraction of the Quercetin flavonoid.

Keywords


1. Kramer SN. First pharmacopeia in man's recorded history. Am J Pharm Sci Support Public Health. 1954; 126(3): 76-84.
2. Saad B, Azaizeh H, Said O. Tradition and perspectives of arab herbal medicine: a review. Evid Based Complement Alternat Med. 2005; 2(4): 475-9.
3. Mozafarian V. Trees and shrubs of Iran. Tehran: Contemporary Culture Pub; 2004.
4. Toky OP, Arya S, Bisht RP. Ecological perspective of Prosopis cineraria (L.) Duce in Arid and Semi-Arid India. In: Dutton RW, ed. Prosopis species, aspects of their value, research and development. Rome: FAO Pub; 1992: 301-9.
5. Al-Qura’n S. Taxonomical and pharmacological survey of therapeutic plants in Jordan. 2008; J Nat Prod. 1(1): 10-26.
6. O'Neil MJ. Merck Index: An Encyclopedia of Chemicals, Drugs and Biologicals. 13th ed. USA: Kaempferol, Merck Research Laboratories Division of Merck Company, Whitehouse Statio; 2001.
7. Middleton E, Kandaswami C. The impact of plant flavonoids on mammalian biology implications for immunity, inflammation and cancer. In: Harborne JB. The flavonoids: Advances in Research Science. Chapman & Hall: London; 1993: 619-52.
8. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996; 20(7): 933-56.
9. Jaimand K. Rezaee MB. Asareh MH. Tabaei Aghdaei SR, Meshkizadeh S. Extraction and determination of Kaempferol and Quercetin in petals of 10 genotypes of Rosa damascena Mill. from western Iran. Iran J Med Arom Plants. 2010; 25(4): 547-55.
10. United States Pharmacopoeia Commission. The United States Pharmacopoeia (USP)-National Farmulary (NF 23). USA: United States Pharmacopeial; 2005: 1500-1.
11. Harzallah-Skhiri F, BenJannet H. Flavonoids diversification in organs of two prosopis farcta (banks & sol.) eig. (leguminosea, mimosoideae) populations occurring in the northeast and the southeast of Tunisia. J Appl Sci Res. 2005; 1(2): 130-36.
12. Gulalp B, Karcioglu O. The first report of Prosopis farcta ingestion in children: is it serious? Int J Clin Pract. 2008 May; 62(5): 829-30.
13. Alston RE, Turner BL. News techniques in analysis of complex natural hybridization. Nat Acad Sc. 1962; 48(2): 130-37.
14. Alston RE. Flavonoid chemistry of Baptisia: a current evaluation of chemical methods in the analysis of inter-specific hybridization. Taxon. 1965; 14(8): 268-74.
15. Levin DA. Chromatographic evidence of hybridization and evolution in Phlox maculate. Amer J Bot. 1966; 53: 238-45.
16. Hsiao J-Y. A numerical taxonomic study of the genus Platanus based on morphological and phenolic characters. Amer J Bot. 1973; 60(7): 678-84.
17. Hunziker JH, Comas C. Larrea interspecific hybrids revisited (Zygophyllaceae). Darwiniana. 2002; 40(1-4): 33-8.
18. Carman NJ. Systematic and ecological investigations in the genus Prosopis (Mimosoceae) emphasizing the natural products chemistry [dissertation]. Austin Texas: University of Texas at Austin, Austin Texas; 1973.
19. Bragg, LH, Bacon JD, Mc-Millan C, Mabry TJ. Flavonoid patterns in the Prosopis juliflora complex. Biochem Sys Ecol. 1978; 6(2): 113-16.
20. Palacios RA, Bravo LD. Hibridacion natural de Prosopis (Leguminosae) en la region chaqueta argentina. Evidenciae morfologicas ycromatograficas. Drawiniana. 1981; 23: 3-35.
21. Ranjbar-Heidari A, Khaiatzadeh J, Mahdavi-Shahri N, Tehranipoor M. The effect of fruit pod powder and aquatic Extract of Prosopis farcta on Healing Cutaneous wounds in diabetic rat. Zahedan J Res Med Sci. 2012; 14 (5):16-20.
22. Afifi F. Hypoglycemic effects of Prosopis farcta. Int J Pharmacognosy. 1993. 31(1): 161-4.
23. Al-Aboudi A, Afifi FU. Plants used for the treatment of diabetes in Jordan: A review of scientific evidence. J Pharmacol Biol. 2011; 49(3): 221-39.